An Alternative Specimen in Toxicological Analysis: Nail

  • Pınar Efeoğlu Özşeker
  • Nebile Dağlıoğlu Çukurova Üniversitesi Tıp Fakültesi Adli Tıp Anabilim Dalı
Keywords: nail, toxicological analysis, interpretation

Abstract

The use of alternative biological samples has increased with the development of sensitive and selective analytical techniques in recent days. Keratinous biological samples such as hair and nail have attracted the attention of clinical and forensic toxicologist when blood and urine sample especially were not taken in cases which were the decomposed and unfolded postmortem cases.

Drugs, medicines, drug of abuse and heavy metals may accumulate for a long time in hair and nail. The nail sample may be useful for toxicological analysis when the hair sample, which is a good marker for past use, is inadequate. Sample preparation and analytical methods to be used are important because of the complex structure of nail and low drug concentration detected in nail.

In this study, the basic structure of nail used as an alternative to toxicological analysis, the mechanism of drug or substance transfer into the nail, analytical methods, the advantages / disadvantages of nails and how to interpret the results will be examined.

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Author Biography

Nebile Dağlıoğlu, Çukurova Üniversitesi Tıp Fakültesi Adli Tıp Anabilim Dalı
Çukurova Üniversitesi Tıp Fakültesi Adli tıp Anabilim Dalı Adli Toksikoloji, Doçent

References

Pragst F, Balikova MA. State of the art in hair analysis for detection of drug and alcohol abuse. Clin Chim Acta,2016; 370:17–49.

Kronstrand R, Förstberg-Peterson S, Kägedal B, Ahlner J, Larson G. Codeine concentration in hair after oral administration is dependent on melanin content, Clin Chem, 1999; 45: 1485–1494.

Testorf MF, Kronstrand R, Svensson SPS, Lundström I, Ahlner J. Characterization of [3-H] flunitrazepam binding to melanin. Anal Biochem, 2001;298:259–264

Cappelle D, Yegles M, Neels H, L. N. van Nuijs A, De Doncker M, Maudens K, Covaci A, Crunelle CL. Nail analysis for the detection of drugs of abuse and pharmaceuticals: a review. Forensic Toxicol, 2015; 33: 12–36.

Lemos NP. Analysis of Bile&Nail as Alternative Biological Specimens in Forensic Toxicology [Doctorate Thesis). University of Glasgow,1996: 23-30.

Jenkins AJ. Drug Testing in Alternate Biological Spicemens. Forensic Science &Medicine, Humana Press, Chapter 3;2007;44-48

Zaias N. The nail in health and disease. MTP Press, Spectrum Publications, 1980 Chapter 1, 1-18 Florida

Palmeri A, Pichini S, Pacifici R, Zuccaro P, Lopez A. Drugs in Nails. Clin Pharmacokinet 2000, 38(2):95-110

Willemsen M, De Doncker P, Willems J, Woestenborghs R, Van de Velde V,Heykants J, Van Cutsem J, Caunwenbergh G, Roseeuw D. Post treatment itraconazole levels in the nail: New implications for treatment in onychomycosis. Journal of American Academy of Dermatology,1992, 26;5(1):731-735.

Gauwenbergh G, Degreef H, Heykants J, Woestenborghs R, Van Rooy P and Haeverans K. Pharmacokinetic profile of orally administered itraconazole in human skin. J. Am. Acad. Dermatol,1988;18: 263-268.

Hay R.J. Pharmacokinetic evaluation of fluconazole in skin and nails. Inter. J.Dermatol,1992;31(2): 6-7.

Faergemann J and Laufen H. Levels of fluconazole in normal and diseased nails during and after treatment of onychomycoseis in toe-nails with fluconazole 150 mg once weekly. Acta Dermatol. Venereol, 1996; 76: 219-221.

Matsumoto T, Tanuma H, Kaneko S, Takasu H and Nishiyarna S. Clinical and pharmacokinetic investigations of oral terbinafine in patients with tinea unguium. Mycoses,1995;38: 135-144.

Schatz F, Brautigam M, Dobrowolski E, 1. Leffendy, Haberl H, Mensing H et al. Nail incorporation kinetics of terbinafine in onychomycosis patients. Clin. Exp.Dermatol,1995; 20: 377-38

Suzuki O, Hattori H, Asano M. Nails as useful materials for detection of methamphetamine or amphetamine abuse. Forensic Sci Int.1984; 24:9–16

Engelhart DA, Lavins ES, Sutheimer CA. Detection of drugs of abuse in nails. J Anal Toxicol, 1998;22:314–318.

Deposition characteristics of metamphetamine and amphetamine in fingernail clippings and hair sections. Journal of Analytical Toxicology,2004;28:411-417

Engelhart DA, Jenkins AJ. Detection of cocaine analytes and opiates in nails from postmortem cases. Journal of Analytical Toxicology,2002;26:489-492

Lemos NP, Anderson RA, Robertson JR. Extraction and determination of cannabis in fingernails by RIA and GC/MS. Journal of Analytical Toxicology,1999

Jones J, Jones M, Plate C, Lewis D (2013) The detection of THCA using 2-dimensional gas chromatography-tandem mass spectrometry in human fingernail clippings: method validation and comparison with head hair. Am J Anal Chem 4:1–8.

Madry MM, Steuer AE, Binz TM, Baumgartner MR and Kraemer T. Systematic investigation of the incorporation mechanisms of zolpidem in fingernails. Drug Testing and Analysis, 2014;6:533-541.

Lemos NP, Anderson RA, Robertson JR. The analysis of methadone in nail clippings from patients in a methadone-maintenance program. Journal of Analytical Toxicology, 2000;24:656-660.

Hang C, Ping X, Min S. Long-term follow-up analysis of zolpidem in fingernails after a single oral dose. Anal Bioanal Chem, 2013;405:7281-7289.

Valente –Campos S, Yonamine M, De Moraes Moreau RL, Silva OA. Validation of a method to detect cocaine and its metabolites in nails by gas chromatography- mass spectrometry. Forensic Science International, 2006;159:218-222.

Irving RC, Dickson SJ.The detection of sedatives in hair and nail samples using tandem LC-MS-MS. Forensic Science International, 2007;166:58-67

Kim JY, Cheong JC, Kim MK, II Lee J, In MK. Simultaneous determination of amphetamine-type stimulants and cannabis in fingernails by gas chromatography-mass spectrometry. Archieves of Pharmacal Research, 2008;31(6):805-813.

Kim JY, Shin SH, In KM. Determination of amphetamine-type stimulants, ketamine and metabolite in fingernails by gas chromatography-mass spectrometry. Forensic Science International, 2010;194:108-114.

Kim JY, Cheong JC, II Lee J, Son JH, In MK. Rapid and simple GC-MS Method for determination of psychotropic phenylalkylamine derivates in nails using micro-pulverized extraction. Journal of Forensic Sciences,2012;57(1):228-233.

Madej KA. Analysis of meconium, nails and tears for determination of medicines and drugs of abuse. Trends in anaytical Chemistry,2010;29(3):246-259.

Krumniegel F, Hastedt M, Westerndorf L, Niebel A, Methling M, Parr MK, Tsokos M. The use of nails as an alternative matrix for the long-term detection of previous drug intake: validation of sensitive UHPLC-MS/MS methods fort he quantitation of 76 substances and comparison of analytical results for drugs in nail and hair samples. Forensic Sci Med Pathol, 2016 DOI:10.1007/s12024-0169801-1

Daniel III CR, Piraccini BM, Tosti A. The nail and hair in forensic science. J Am Acad Dermatol, 2004;50(2):258-261

Published
2018-04-05
How to Cite
Efeoğlu Özşeker, P., & Dağlıoğlu, N. (2018). An Alternative Specimen in Toxicological Analysis: Nail. The Bulletin of Legal Medicine, 23(1), 47-52. https://doi.org/10.17986/blm.2017332889
Section
Reviews