Detection of Prostate Spesific Antigen P30 for Forensic Purpose by Using the Ouchterlony Technique Double Diffusion in Agar
DOI:
https://doi.org/10.17986/blm.199831286Keywords:
sexual assaults, rape, semen. prostat specific antigen, ouchterlony techniqueAbstract
Forensic sciences have important role in evaluation of the medical and physical evidences of sexual assaults. Genital and anal physical findings of the victims are mostly suggestive but not diagnostic. The only specific physical finding of genital trauma is acute laceration of the hymen. Obvious indicators for sexual assaults are the presence of sperms or other seminal components on the bodies or/and the cloths of the victims or at the crime scene. Cytological, immunological and biochemical methods have been used for identification of seminal components. Prostate specific antigen (PSA) has been recognized as a forensic marker because of its biological specificity and detectability in trace amounts, and stability in dried stains and vaginal environment. Nowadays, simple and high technological methods are available for detection of PSA. In this study, a simple immunological method (Ouchterlony technique) has been modified and adapted for our serology laboratory as a preliminary detection method of PSA. The performed experiments revealed that the best precipitation zones were obtained with the 1/2 (15ml) dilution of anti-PSA and with the 1/40 and 1/80 dilutions of the seminal plasma (10ml). The vaginal swabs and the extractions of swabs should be tested simultaneously for the best results.
Key words: Sexual assaults, Rape, Semen. Prostat specific antigen, Ouchterlony technique.
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